Diagnosis of mixed gastrointestinal nematode infection in goat by an indirect-ELISA

Indirect ELISA for diagnosis of Brucella ovis infection in rams

Brucella ovis is a major cause of epididymitis in sexually mature rams, resulting in subfertility, infertility, and economic losses for the sheep industry worldwide. The aim of this study was to develop an indirect ELISA (iELISA) using recombinant proteins, namely rBoP59 and rBP26, as antigens for serological diagnosis of B. ovis infection. The BoP59 and BP26 recombinant proteins were expressed...

Immunodiagnosis of Haemonchus contortus infection in sheep by indirect enzyme linked immunosorbent assay (ELISA)

Indirect plate enzyme-linked immunosorbent assay was standardized and evaluated for its effectivenessin immunodiagnosis of haemonchosis in experimental and clinical cases in sheep by using somatic wholeadult antigen of H. contortus. Plate ELISA was standardized using 5 μg/well antigen concentration with1:100 and 1:1000 of sera and conjugate dilution. Indirect plate ELISA was able to demonstrate...

Gastrointestinal Nematode Infection

standardization of an indirect elisa for serological diagnosis of aspergilloma

conclusions: it was concluded that the elisa is a suitable method for large scale screening of antibodies against aspergilloma. the easy techniques have shortened the required time of tests (approximately 1.30 hours for our elisa, as opposed to 2.30 hours with commercial elisa kit). this may be a substantial benefit for large numbers of samples that should be tested. materials and methods: an i...

An explicit immunogenetic model of gastrointestinal nematode infection in sheep

Gastrointestinal nematodes are a global cause of disease and death in humans, wildlife and livestock. Livestock infection has historically been controlled with anthelmintic drugs, but the development of resistance means that alternative controls are needed. The most promising alternatives are vaccination, nutritional supplementation and selective breeding, all of which act by enhancing the immu...

Comparison of viral RNA electrophoresis and indirect ELISA methods in the diagnosis of human rotavirus infection.

It has been estimated that roughly 420,000 children under five years of age die annually in Latin America, most of the victims being less than a year old. Diarrhea1 disease syndromes are believed responsible for roughly one-fifth of these deaths (I). The numbers of actual diarrhea1 disease cases reported have been relatively low (on the order of 250,000 cases per year) because of statistical un...